Enhanced Petroleum Removal by Potent Biosurfactant Producer Bacillus Subtilis CC9 Strain Isolated from an Oil Field

This study aimed to enhance the removal of petroleum with potent biosurfactant producing bacterial strains (Bacillius subtilis CC9, Enterobacter asburiae WMB1, Klebsiella oxytoca Rizhao 536-1, and Acinetobacter pittii PgBE252) isolated from an oil field. The production was determined by different screening methods such as hemolytic activity, drop-collapse, spreading, BATH, emulsification index surface tension. While presence detected individual strain, B. CC9 strain found be quite effective on degradation (92%) a high yield (0.95 ±0.05 g L-1). rate constant half-life period were calculated K = 2.533 day−1, t1/2 0.273. It has been clearly shown GC/MS analysis that long chain n-alkanes (C31 - C35) well short medium chains. FT-IR also approved aliphatic peptide moieties extracted strain. is remarkably pointed out this can used in advanced bioremediation studies alternative chemical surfactants.